Properties
Description
Recombinant endoglycoceramidase I is a glycolipid-specific hydrolase derived from Rhodococcus equi and its recombinant protein is expressed in Rhodococcus erythropolis.
Unit Definition
One unit of EGCase I will hydrolyze 1 μmol of ganglioside GM1 per minute at pH 5.5 at 37°C.
Expression System
Rhodococcus erythropolis
Reaction Conditions
50 mM Sodium acetate containing 0.4% Triton X-100, pH 5.5
Production Methods
Fermentation
Detection Method
The mixture of recombinant endoglycoceramidase I and 100 nmol of GM1 in 50 μL is incubated at 37°C for 15 min. Quantification of the reducing end of the released oligosaccharides is determined using the modified Somogyi-Nelson method. Glucose is determined by a modified Somogyi-Nelson method.
Sensitivity
Heat sensitive
Concentration
300 mU per vial (6 mU/μL)
Form
Colorless to almost colorless clear liquid
Buffer
20 mM Sodium phosphate, 50 mM NaCl buffer, pH 7.0
Applications
Recombinant endoglycoceramidase I can be used to cleave glycosidic linkages between oligosaccharides and ceramides of various glycosphingolipids (GSLs) under conditions that require detergent or cofactor activator II.
