Properties
Description
Recombinant endoglycoceramidase II is a glycolipid-specific hydrolase derived from Rhodococcus sp. and its recombinant protein is expressed in Rhodococcus erythropolis.
Unit Definition
One unit will hydrolyze 1 μmol of ganglioside GM1 per minute at pH 5.5 at 37°C.
Expression System
Rhodococcus erythropolis
Reaction Conditions
50 mM Sodium acetate containing 0.4% Triton X-100, pH 5.5
Production Methods
Fermentation
Detection Method
The mixture of recombinant endoglycoceramidase II and 100 nmol of GM1 in 50 μL is incubated at 37°C for 15 min. Quantification of the reducing end of the released oligosaccharides is determined using the modified Somogyi-Nelson method. Glucose is determined by a modified Somogyi-Nelson method.
Sensitivity
Heat sensitive
Concentration
100 mU per vial (2 mU/μL)
Form
Colorless to almost colorless clear liquid
Buffer
20 mM Sodium phosphate, 50 mM NaCl buffer, pH 7.0
Applications
Recombinant endoglycoceramidase II can be used to cleave the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs).
