Properties
Description
Recombinant Alicyclobacillus acidocaldarius β-galactosidase 42A produced by Escherichia coli, is a crucial enzyme in the human body and is responsible for hydrolyzing galactosides into galactose.
Expression System
Escherichia coli
Species
Alicyclobacillus acidocaldarius
Purity
≥90%, determined by SDS-PAGE.
Activity
β-Galactosidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Alicyclobacillus acidocaldarius β-galactosidase 42A is applied for the structural research of sugar, the measurement of lactose, and as an enzyme label for enzyme immunoassay. It has the ability to hydrolyze 2-nitrophenyl-β-D-fucoside, 2-nitrophenyl-β-D-galactoside, 4-nitrophenyl-α-L-arabinoside.
