Properties
Description
Recombinant Bacillus cereus chitosanase 8B produced by Escherichia coli, functions as an endo-1,4-β-chitosanase, which cleaves the 1,4-β-glycosidic bonds within chitosan molecules.
Expression System
Escherichia coli
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus cereus chitosanase 8B can be used for its great potential value. It has the ability to hydrolyze colloidal chitosan and soluble chitosan.
