Properties
Description
Recombinant Bacillus halodurans acetyl xylan esterase 12A produced by Escherichia coli, functions as an enzyme produced in Escherichia coli. It exhibits deacetylase activity on the N-acetyl moiety of N-acetylglucosamine, diacetylchitobiose, and triacetylchitotriose.
Expression System
Escherichia coli
Species
Bacillus halodurans
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetyl xylan esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus halodurans acetyl xylan esterase 12A can be used in carbohydrate research and biofuel industries. It participates in the de-esterification of 1,4-β-xylans, 4-nitrophenyl acetate, 7-aminocephalosporanic acid, cephalosporin C, pentaacetate, β-naphthyl acetate.
