Properties
Description
Recombinant Bacillus licheniformis α-phosphotrehalase 13A produced by Escherichia coli, belongs to the glycoside hydrolase family 13 (GH13) and consists of a single domain.
Expression System
Escherichia coli
Species
Bacillus licheniformis
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus licheniformis α-phosphotrehalase 13A can be used for glycobiology research. It has the ability to hydrolyze p-nitrophenyl-α-D-glucopyranoside (pNPG), trehalose-6-phosphate.
