Properties
Description
Recombinant Bacillus subtilis acetyl xylan esterase 12A1 produced by Escherichia coli, is a recombinant enzyme produced in Escherichia coli. It possesses enzyme commission (E.C.) number 3.5.1.25 and facilitates the hydrolysis of the acetyl group from GlcNAc-6-P.
Expression System
Escherichia coli
Species
Bacillus subtilis
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetyl xylan esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus subtilis acetyl xylan esterase 12A1 can be used in carbohydrate research and biofuel industries. It participates in the de-esterification of 4-nitrophenyl acetate, 7-aminocephalosporanic acid, acetylated xylan, cephalosporin C, pentaacetate, and β-naphthyl acetate.
