Properties
Description
Recombinant Bacillus subtilis acetyl xylan esterase 7A1 produced by Escherichia coli, is responsible for catalyzing the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, and acetylated glucose. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Bacillus subtilis
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetyl xylan esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus subtilis acetyl xylan esterase 7A1 can be used in carbohydrate research and biofuel industries. It participates in the de-esterification of 1,2,3,4-tetra-O-acetyl-xylopyranose, 2,3,2′,3′,4′-penta-acetyl-xylobiose, 3′-O-acetyl-2′-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, cephalosporin C.
