Properties
Description
Recombinant Bacillus subtilis polygalacturonase 4A produced by Escherichia coli, has the E.C. number 3.2.1.67 and is an exo-1,4-α-polygalacturonase enzyme.
Expression System
Escherichia coli
Species
Bacillus subtilis
Purity
≥90%, determined by SDS-PAGE.
Activity
Polygalacturonases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus subtilis polygalacturonase 4A can be used in research for food, beverage, feed, textile, and paper industries. It has the ability to hydrolyze 4-nitrophenyl-α–1,4-di-galacturonate, polygalacturonans.
