Properties
Description
Recombinant Bacillus sp. inulin fructotransferase 91A produced by Escherichia coli, functions as an enzyme that produces α-D-fructofuranose β-D-fructofuranose 1,2’:2,3’-dianhydride (DFA III) by sequentially removing the (2->1)-β-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
Expression System
Escherichia coli
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacillus sp. inulin fructotransferase 91A can be used in research for food and pharmaceutical industries as a dietary fiber and prebiotic. It has the ability to hydrolyze inulin oligosaccharides.
