Properties
Description
Recombinant Bacteroides fragilis mannosylglucose phosphorylase 130A produced by Escherichia coli, is classified under the enzyme commission (E.C.) number 2.4.1.281.
Expression System
Escherichia coli
Species
Bacteroides fragilis
Purity
≥90%, determined by SDS-PAGE.
Activity
Mannosylglucose phosphorylases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides fragilis mannosylglucose phosphorylase 130A can be used in enzymatic synthesis of novel manno-oligosaccharides. It has the ability to hydrolyze 4-O-β-D-mannosyl-D-glucose.
