Properties
Description
Recombinant Bacteroides fragilis sialidase 33A produced by Escherichia coli, is responsible for hydrolyzing terminal N- or O-acetylneuraminic acids which are α2,3-, α2,6-, α2,8- linked to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids.
Expression System
Escherichia coli
Species
Bacteroides fragilis
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides fragilis sialidase 33A can be used in clinical chemistry, glycobiology, and carbohydrate research. It has the ability to hydrolyze colonic acid, glycolipids, glycoproteins, sialic acids, and synthetic substrates.
