Properties
Description
Recombinant Bacteroides plebeius β-agarase 118A produced by Escherichia coli, is responsible for cleaving the core of agarose polysaccharides into new agar oligosaccharides.
Expression System
Escherichia coli
Species
Bacteroides plebeius
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides plebeius β-agarase 118A can be used to recover DNA or RNA fragments from low-melting-point agarose for amplification, cloning, or sequencing. It has the ability to hydrolyze agarose.
