Properties
Description
Recombinant Bacteroides stercoris chondroitin AC lyase 8A produced by Escherichia coli, is a kind of heparin-degrading enzyme produced in Escherichia coli. It is responsible for cleaving 1→4 linkages between hexosamines and uronic acid residues to produce disaccharides.
Expression System
Escherichia coli
Species
Bacteroides stercoris
Purity
≥90%, determined by SDS-PAGE.
Activity
Chondroitin lyases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides stercoris chondroitin AC lyase 8A can be used in various industries and participates in the eliminative cleavage of chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C), hyaluronic acid (hyaluronan).
