Properties
Description
Recombinant Bacteroides thetaiotaomicron Δ-4,5-unsaturated β-glucuronyl hydrolase 105A produced by Escherichia coli, is an enzyme that has the ability to cleave the glycosidic linkage between Δ-4,5-unsaturated uronic acid and GlcN/GlcNAc disaccharides.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Δ-4,5-Unsaturated β-glucuronyl hydrolases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron Δ-4,5-unsaturated β-glucuronyl hydrolase 105A can be used in carbohydrate and biofuel research. It has the ability to hydrolyze homogalacturonan oligosaccharides.
