Properties
Description
Recombinant Bacteroides thetaiotaomicron chondroitin ABC lyase 8B produced by Escherichia coli, catalyzes the cleavage of endo-1,4-β-galactosaminic bonds between acetylgalactosamine and either glucuronic or iduronic acid.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Chondroitin lyases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron chondroitin ABC lyase 8B can be used in various biotechnological and biomedical applications. It participates in the eliminative cleavage of chondroitin sulfate A (CS-A), chondroitin sulfate B (CS-B), chondroitin sulfate C (CS-C), chondroitin (C), and H.
