Properties
Description
Recombinant Bacteroides thetaiotaomicron fucosidase 141A produced by Escherichia coli, is a glycosidase that hydrolyzes the α-1,6-linked fucose relating to the reducing-end N-acetylglucosamine on glycoproteins.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron fucosidase 141A can be used for glycobiology research. It has the ability to hydrolyze rhamnogalacturonan II.
