Properties
Description
Recombinant Bacteroides thetaiotaomicron α-galactosidase 97B produced by Escherichia coli, is a homodimeric glycoprotein that catalyzes the hydrolysis of terminal α-galactosyl moieties of oligosaccharides and polysaccharides.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
α-Galactosidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron α-galactosidase 97B can be used for glycobiology research. It has the ability to hydrolyze galactomannas.
