Properties
Description
Recombinant Bacteroides thetaiotaomicron glucoamylase 97A produced by Escherichia coli, hydrolyses the residues from the ends of the polymers, such as starch.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron glucoamylase 97A is useful for the saccharification of partially processed starch/dextrin to glucose, which is widely useful for numerous fermentation processes. It has the ability to hydrolyze 1,4-α-glucans.