Properties
Description
Recombinant Bacteroides thetaiotaomicron α-glucosidase 31A produced by Escherichia coli, is an exoenzyme and is responsible for hydrolyzing (1→4)-linked α-D-glucose residues with the release of D-glucose.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron α-glucosidase 31A can be used in carbohydrate and biofuel research. It has the ability to hydrolyze isomaltose, malto-oligosaccharides, and maltose.