Properties
Description
Recombinant Bacteroides thetaiotaomicron heparin lyase 15A produced by Escherichia coli, specifically cleaves the bond between 3-sulfated rhamnose (Rha3S) and either D-glucuronic acid (GlcA) or L-iduronic acid (IduA) in the marine polysaccharide levan. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron heparin lyase 15A can be used for the structural analysis of glycosaminoglycans (GAGs) and participates in the eliminative cleavage of heparan sulfate, heparin.
