Properties
Description
Recombinant Bacteroides thetaiotaomicron heparin-sulfate lyase 12A produced by Escherichia coli, is a kind of heparin-degrading enzyme produced in Escherichia coli. It is responsible for cleaving 1→4 linkages between hexosamines and uronic acid residues to produce disaccharides.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron heparin-sulfate lyase 12A can be used in various biotechnological and biomedical fields and participates in the eliminative cleavage of heparan sulfate.
