Properties
Description
Recombinant Bacteroides thetaiotaomicron exo-α-galacturonidase 28E produced by Escherichia coli, belongs to the glycoside hydrolase family 28 (GH28) and specifically targets rhamnogalacturonan I.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Galacturonidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron exo-α-galacturonidase 28E can be used for enzymatic degradation of rhamnogalacturonan I. It has the ability to hydrolyze rhamnogalacturonan I.