Properties
Description
Recombinant Bacteroides thetaiotaomicron β-N-acetylglucosaminidase 84A produced by Escherichia coli, is responsible for the hydrolysis of nonreducing terminal N-acetyl-D-hexosamine residues.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetylglucosaminidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron β-N-acetylglucosaminidase 84A can be used in the hydrolysis of O-GlcNAc modifications on proteins. It has the ability to hydrolyze O-acetylglucosamine (O-GlcNAc).
