Properties
Description
Recombinant Bacteroides thetaiotaomicron l-rhamnose-α-1,4-D-glucuronate lyase 42A produced by Escherichia coli, catalyzes the cleavage of L-Rha-α1,4–D-GlcA linkages in gum arabic (GA) and related polysaccharides.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Rhamnoglucuronate lyases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron l-rhamnose-α-1,4-D-glucuronate lyase 42A can be used for glycobiology research and participates in the eliminative cleavage of L-Rha-1,4-α-D-GlcA.
