Properties
Description
Recombinant Bacteroides thetaiotaomicron β-mannosidase 2A produced by Escherichia coli, is responsible for the enzymatic cleavage of β-1,4-mannans and plays a key role in the germination of seeds and microbial hemicellulose degradation.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron β-mannosidase 2A can be used in industrial and consumer production. It has the ability to hydrolyze 4-nitrophenyl-α-glucopyranoside (GlcpbNp), 4-nitrophenyl-β-D-mannopyranoside, arabinoxylans, xylose.
