Properties
Description
Recombinant Bacteroides thetaiotaomicron α-mannosidase 92E produced by Escherichia coli, is a glycoside hydrolase and is responsible for cleaving the alpha-mannose.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron α-mannosidase 92E can be used for the digestion of neutral glycans. It has the ability to hydrolyze mammalian high mannose N-glycans (HMNG), Man9GlcNAc2.
