Recombinant Bacteroides thetaiotaomicron pectate lyase 1D

Synonym: BtPel1D; Polygalacturonic transeliminase; Pectic acid transeliminase; Polygalacturonate lyase; Endopectin methyltranseliminase.

Enzyme Commission Number: 4.2.2.2

Product Size

0.5 mg; 1 mg; 1.5 mg; 2 mg

Price

Datasheet

MSDS

Properties

Description

Recombinant Bacteroides thetaiotaomicron pectate lyase 1D produced by Escherichia coli, is responsible for plant cell wall decomposition by cleaving pectin through a β-elimination mechanism. It is produced in Escherichia coli.

Expression System

Escherichia coli

Species

Bacteroides thetaiotaomicron

Concentration

0.5 mg/mL

Form

Liquid

Purity

≥90%, determined by SDS-PAGE.

Activity

Pectate lyases

Buffer

35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl₂, and 25% (v/v) glycerol

Applications

Recombinant Bacteroides thetaiotaomicron pectate lyase 1D can be used for pectin measurement and carbohydrate research and participates in the eliminative cleavage of polygalacturonic acid (PGA).