Properties
Description
Recombinant Bacteroides thetaiotaomicron pectin methylesterase NC produced by Escherichia coli, acts as an enzyme involved in the demethylation process of pectins.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Pectin methylesterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron pectin methylesterase NC can be used for the demethylation of pectins for various purposes. It participates in the de-esterification of methylated pectins.