Properties
Description
Recombinant Bacteroides thetaiotaomicron RG galacturonohydrolase 28C produced by Escherichia coli, catalyzes the exohydrolysis of the α-D-GalA-1,2-α-L-Rha bond in rhamnogalacturonan oligosaccharides, releasing D-galacturonic acid from the non-reducing end with the inversion of configuration.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Galacturonohydrolases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron RG galacturonohydrolase 28C can be used in research for food processing, dietary supplements, and biotechnology. It hydrolyzes the D-GalA-α2-L-Rha linkage in rhamnogalacturonan I (RGI). It has the ability to hydrolyze rhamnogalacturonan I.
