Properties
Description
Recombinant Bacteroides thetaiotaomicron RGI acetyl esterase NC1 produced by Escherichia coli, plays a role in cleaving the ester link between the polysaccharide main chain of xylans and monomeric or dimeric ferulates. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Pectin acetyl esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron RGI acetyl esterase NC1 can be used in food processing research, pharmaceuticals, and biotechnology. It participates in the de-esterification of acetylated rhamnogalacturonan I (RGI).
