Properties
Description
Recombinant Bacteroides thetaiotaomicron α-rhamnosidase 78B & Kdo hydrolase 33A produced by Escherichia coli, is responsible for hydrolyzing the terminal α-L-rhamnose from several natural rhamnoglycosides.
Expression System
Escherichia coli
Species
Bacteroides thetaiotaomicron
Purity
≥90%, determined by SDS-PAGE.
Activity
Rhamnogalacturonases & Other Activities
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bacteroides thetaiotaomicron α-rhamnosidase 78B & Kdo hydrolase 33A possesses several uses, such as debittering citrus juices in the research for the food industry. It has the ability to hydrolyze 4-nitrophenyl-α-L-rhamnose, and rhamnogalacturonan II.
