Properties
Description
Recombinant Bifidobacterium longum α-galactosidase 36A produced by Escherichia coli, is a homodimeric glycoprotein that catalyzes the hydrolysis of terminal α-galactosyl moieties of oligosaccharides and polysaccharides.
Expression System
Escherichia coli
Species
Bifidobacterium longum
Purity
≥90%, determined by SDS-PAGE.
Activity
α-Galactosidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bifidobacterium longum α-galactosidase 36A can be used for glycobiology research. It has the ability to hydrolyze 4-nitrophenyl-α-D-galactopyranoside, α-galactose containing polysaccharides.
