Properties
Description
Recombinant Bifidobacterium longum endo-α-N-acetylgalactosaminidase 101A produced by Escherichia coli, functions in catalyzing the release of Gal-1,3-β-GalNAc α-linked to serine or threonine residues of mucin-type glycoproteins.
Expression System
Escherichia coli
Species
Bifidobacterium longum
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetylgalactosaminidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Bifidobacterium longum endo-α-N-acetylgalactosaminidase 101A can be used in research, biotechnology, and therapeutic interventions. It has the ability to hydrolyze GalNAcα1-pNP, Mucin-type O-glycans.
