Recombinant Capnocytophaga canimorsus β-acetylglucosaminidase 18A

Synonym: CcAcp18A; Mannosyl-glycoprotein endo-β-N-acetylglucosaminidase

Enzyme Commission Number: 3.2.1.96

Product Size

0.25 mg; 0.50 mg; 0.75 mg; 1 mg

Price

Datasheet

MSDS

Properties

Description

Recombinant Capnocytophaga canimorsus β-acetylglucosaminidase 18A produced by Escherichia coli, is an enzyme that is responsible for hydrolyzing the diacetylchitobiosyl unit in glycopeptides and glycoproteins with the (Man(GlcNAc)(2))Asn structure, releasing the intact oligosaccharide except for one N-acetyl-D-glucosamine residue that remains attached to the protein.

Expression System

Escherichia coli

Species

Capnocytophaga canimorsus

Concentration

0.25 mg/mL

Form

Liquid

Purity

≥90%, determined by SDS-PAGE.

Activity

Acetylglucosaminidases

Buffer

35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl₂, and 25% (v/v) glycerol

Applications

Recombinant Capnocytophaga canimorsus β-acetylglucosaminidase 18A can be used for glycobiology research. It has the ability to hydrolyze glycoproteins.