Properties
Description
Recombinant Catenovulum agarivorans ulvan lyase 25B produced by Escherichia coli, specifically cleaves the bond between 3-sulfated rhamnose (Rha3S) and either D-glucuronic acid (GlcA) or L-iduronic acid (IduA) in the marine polysaccharide ulvan.
Expression System
Escherichia coli
Species
Catenovulum agarivorans
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Catenovulum agarivorans ulvan lyase 25B can be used for glycobiology research and participates in the eliminative cleavage of ulvan.
