Properties
Description
Recombinant Clostridium cellobioparum glucuronyl esterase 15A produced by Escherichia coli, targets the ester bonds between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.
Expression System
Escherichia coli
Species
Clostridium cellobioparum
Purity
≥90%, determined by SDS-PAGE.
Activity
Glucuronyl esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium cellobioparum glucuronyl esterase 15A can be used in carbohydrate research and research for the food industry. It participates in the de-esterification of glucuronyl crosslinks between hemicelluloses and lignin.
