Properties
Description
Recombinant Clostridium cellulolyticum cellodextrinase 1A produced by Escherichia coli, is classified with the enzyme commission (E.C.) number 3.2.1.74 and functions as an exo-1,4-β-glucosidase/glucanase.
Expression System
Escherichia coli
Species
Clostridium cellulolyticum
Purity
≥90%, determined by SDS-PAGE.
Activity
Cellodextrinases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium cellulolyticum cellodextrinase 1A can be used for glycobiology research. It has the ability to hydrolyze 4-nitrophenyl-β-D-glucopyranoside (PNPG), cellobiose, cellotetraose, cellotriose, lactose, o-nitrophenyl-β-galactopyranoside.
