Properties
Description
Recombinant Clostridium cellulolyticum β-mannanase 5B produced by Escherichia coli, is responsible for the enzymatic cleavage of β-1,4-mannans and plays a key role in the germination of seeds and microbial hemicellulose degradation.
Expression System
Escherichia coli
Species
Clostridium cellulolyticum
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium cellulolyticum β-mannanase 5B can be used in industrial and consumer production. It has the ability to hydrolyze gum, low substituted galactomannans.
