Properties
Description
Recombinant Clostridium perfringens endo-α-N-acetylgalactosaminidase 101A produced by Escherichia coli, specifically catalyzes the release of gal-1,3-β-galNAc that is α-linked to serine or threonine residues in mucin-type glycoproteins.
Expression System
Escherichia coli
Species
Clostridium perfringens
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetylgalactosaminidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium perfringens endo-α-N-acetylgalactosaminidase 101A can be used for its potential implications in studying glycoprotein structures and functions in various biological processes. It has the ability to hydrolyze serine glycoproteins and threonine glycoproteins.
