Properties
Description
Recombinant Clostridium perfringens N-acetylglucosamine endo-β-galactosidase produced by Escherichia coli, has the capability to release GlcNAc-1,4-α-galactose.
Expression System
Escherichia coli
Species
Clostridium perfringens
Purity
≥90%, determined by SDS-PAGE.
Activity
β-Galactosidases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium perfringens N-acetylglucosamine endo-β-galactosidase can be used in the biotechnology and pharmaceutical industries for the enzymatic modification. It has the ability to hydrolyze GlcNAc-α-1,4-Gal-β-1,3-GalNAc-α1-Ser/Thr.