Properties
Description
Recombinant Clostridium perfringens α-mannosidase 125A produced by Escherichia coli, is a glycoside hydrolase and is responsible for cleaving the alpha-mannose.
Expression System
Escherichia coli
Species
Clostridium perfringens
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium perfringens α-mannosidase 125A can be used for the digestion of neutral glycans. It has the ability to hydrolyze 2,4-dinitrophenyl-α-D-mannopyranoside.
