Properties
Description
Recombinant Clostridium phytofermentans 1,3-β-galactosyl-N-acetylhexosamine phosphorylase 112A produced by Escherichia coli, is classified with the enzyme commission (E.C.) number 2.4.1.211 and functions as a β-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine phosphorylase.
Expression System
Escherichia coli
Species
Clostridium phytofermentans
Purity
≥90%, determined by SDS-PAGE.
Activity
Galactosyl-N-acetylhexosamine phosphorylases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium phytofermentans 1,3-β-galactosyl-N-acetylhexosamine phosphorylase 112A can be used for glycobiology research. It has the ability to hydrolyze galacto-N-biose, lacto-N-biose.
