Properties
Description
Recombinant Clostridium phytofermentans nigerose phosphorylase 65A produced by Escherichia coli, belongs to the glycoside hydrolase family 65 (GH65) and consists of a single domain.
Expression System
Escherichia coli
Species
Clostridium phytofermentans
Purity
≥90%, determined by SDS-PAGE.
Activity
Other Activities
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium phytofermentans nigerose phosphorylase 65A can be used in the enzymatic transfer of sugar groups. It has the ability to hydrolyze kojibiose and nigerose.
