Properties
Description
Recombinant Clostridium thermocellum acetyl xylan esterase 2A produced by Escherichia coli, is responsible for catalyzing the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, and acetylated glucose.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Activity
Acetyl xylan esterases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum acetyl xylan esterase 2A can be used in carbohydrate research and biofuel industries. It participates in the de-esterification of 4-nitrophenyl acetate, acetylated xylan, galactoglucomannans, and glucomannan.
