Properties
Description
Recombinant Clostridium thermocellum carbohydrate-binding module 6C produced by Escherichia coli, with ligand specificity of 1,3-β-linked glucooligosaccharide, 1,4-β-linked glucooligosaccharide, 1,6-β-linked glucooligosaccharide, mannans, mannooligosaccharides, is a type of enzyme protein produced in Escherichia coli. It has multiple modules that specifically bind to carbohydrate substrates in order to carry out their function.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Carbonhydrate Specificity
Arabinogalactans, decorated 1,4-β-xylans, undecorated 1,4-β-xylans
Purity
≥90%, determined by SDS-PAGE.
Activity
Carbohydrate binding modules
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum carbohydrate-binding module 6C can be used for glycobiology research.
