Properties
Description
Recombinant Clostridium thermocellum cellobiohydrolase 48A produced by Escherichia coli, is an exocellulase that resolves cellulose by hydrolyzing the 1,4-β-D-glycosidic bonds.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Activity
Cellobiohydrolases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum cellobiohydrolase 48A can be used in research for the textiles, food, feed, carbohydrate, and biofuel industries. It has the ability to hydrolyze avicel, crystalline cellulose, and phosphoric acid-swollen cellulose (PASC).
