Properties
Description
Recombinant Clostridium thermocellum cellobiohydrolase 5A produced by Escherichia coli, is an exocellulase that resolves cellulose by hydrolyzing the 1,4-β-D-glycosidic bonds.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Activity
Cellobiohydrolases
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum cellobiohydrolase 5A can be used in research for the textiles, food, feed, carbohydrate, and biofuel industries. It has the ability to hydrolyze 1,3-1,4-β-glucans, cellodextrins, cellulosic carbohydrates, and crystalline cellulose.
