Properties
Description
Recombinant Clostridium thermocellum cellulase 48A produced by Escherichia coli, possesses broad exo-1,3/1,6-β-glucanase specificity and endo-β-1,4-glucanase activity.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum cellulase 48A can be used in research for textile, animal food, pharmaceutical, detergent, and paper processing industries. It has the ability to hydrolyze amorphous cellulose, and phosphoric acid swollen cellulose (PASC).