Properties
Description
Recombinant Clostridium thermocellum cellulase 8A1 mini-cellulosome produced by Escherichia coli, is involved in the cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds in rhamnogalacturonan I domains within pectin. It is produced in Escherichia coli.
Expression System
Escherichia coli
Species
Clostridium thermocellum
Purity
≥90%, determined by SDS-PAGE.
Activity
Mini-cellulossomes
Buffer
35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol
Applications
Recombinant Clostridium thermocellum cellulase 8A1 mini-cellulosome can be used in biofuel production, textile, and paper manufacturing. It has the ability to hydrolyze cellulosic carbohydrates.
